Indentification of fir plus tree clones based on clone archiv in Mary El Republic

Sheykina O.V., Prohorova A.A, Prohorova E.V.

Mary state technical university (Yoshkar-Ola)

One of the ways to preserve a valuable genetic fund of tree species is to create archives of plus tree clones. Fir clone archive in Mary El Republic was created by planting of grafted saplings in 1985. Twenty plus tree clones selected in natural republic plantings were used to found the archive. For further certification of the object, there was a necessity in specification of the mixed clone scheme. Researches on identification of properties were conducted for this purpose. At the first stage of researches identification of clones was pursued according to morphological character, the analysis of which allows to draw the following conclusions. The archive includes two types of furtrees: 1) according to cone colouring - bright red cones, red cones and green cones; 2) according to the type of branches: comb-shaped, brush-shaped and compact. Cone size of different grafted saplings varies from 6.2 to 10.5 cm in length and from 1.6 to 2.9 cm in width. Metameric variability of these indicators in a crown is characterized as low-up to 15 % (according to S. А. Mamayev). The weight of 1000 seeds varies from 3.65 to 7.16 grams and seed output from cones -from 3.8 to 8.1%. The length of a seed varies from 3.1 to 5.0 mm, the width of seed - from 1.9 to 2.9 mm, the length of a wing - from 7.0 to 13.0 mm, the width of a wing - from 4.9 to 7.2 mm. Generalization of the results shows that the use of all listed above characters is not enough for clone property identification. It is known that isoenzymic polymorphism is formed in an organism under the control of genes; hence, genetically identical plants give identical spectra of enzyme strips. Therefore at the second stage enzymes of seed endosperms were investigated by a method of vertical electrophoresis in polyacrylamide gel. For identification of clones 10 enzyme systems were used: ADH, PGM, GDH, AAT, 6-PGD, DIA, MDH, SKDH, IDH, G-6-PD. As a result of the analysis the genetic clone differentiation was detected that allows to use isoenzymic polymorphism for clonal property identification.

Note. Abstracts are published in author's edition